This presentation discusses a case study for leveraging AI-based knowledge engagement techniques for development and use of Immunization Information Systems (IIS) guidelines. The knowledge base developed by the IIS community is voluminous and growing rapidly, resulting in a valuable yet difficult to navigate resource. Initial results of employing a generative AI tool (ChatGPT) at the CDC’s National Center for Immunization and Respiratory Diseases (NCIRD) are promising and indicate increased accessibility and discoverability of information. By making all guidance documents searchable at once, discoverability and presentation of content are enhanced. The AI tool uses a limited set of trusted in-house sources: guidance manuals, community standards, emails, and meeting notes. This ensures accuracy of responses and guarantees information is only from verified sources. Additional capabilities include support for new content development with information synthesis across multiple resources.